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KMID : 0613820080180121764
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Journal of Life Science
2008 Volume.18 No. 12 p.1764 ~ p.1770
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Enhanced Production of Astaxanthin by Metabolic Engineered Isoprenoid Pathway in Escherichia coli
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Lee Jae-Hyung
Kim Young-Tae
Seo Yeong-Bae
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Abstract
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The goal of this study is to increase production of astaxanthin in recombinant Escherichia coli by engineered isoprenoid pathway. We have previously reported structural and functional analysis of the astaxanthin biosynthesis genes from a marine bacterium, Paracoccus haeundaensis. The carotenoid biosynthesis gene cluster involved in astaxanthin production contained six carotenogenic genes (crtW, crtZ, crtY, crtI, crtB, and crtE genes) and recombinant E. coli harboring six carotenogenic genes from P. haeundaensis produced 400 ㎍/g dry cell weight (DCW) of astaxanthin. In order to increase production of astaxanthin in recombinant E. coli, we have cloned 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (lytB), farnesyl diphosphate (FPP) synthase (ispA), and isopentenyl (IPP) diphossphate isomerase (idi) in the isoprenoid pathway from E. coli and coexpressed these genes in recombinant E. coli harboring the astaxanthin biosynthesis genes. This engineered E. coli strain containing both isoprenoid pathway gene and astaxanthin biosynthesis gene cluster produced 1,200 ㎍/g DCW of astaxanthin, resulting 3-fold increased production of astaxanthin.
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KEYWORD
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Astaxanthin, carotenoid, isoprenoid, Paracoccus haeundaensis
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